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Review Article

Gene editing in tomatoes

Joyce Van Eck
Emerging Topics in Life Sciences Nov 10, 2017, 1 (2) 183-191; DOI: 10.1042/ETLS20170056
Joyce Van Eck
The Boyce Thompson Institute, 533 Tower Road, Ithaca, NY 14853, U.S.A.Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, U.S.A.
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Abstract

Tomato is an effective model plant species because it possesses the qualities necessary for genetic and functional studies, but is also a food crop making what is learned more translatable for crop improvement when compared with other non-food crop models. The availability of genome sequences for many genotypes and amenability to transformation methodologies (Agrobacterium-mediated, direct DNA uptake via protoplasts, biolistics) make tomato the perfect platform to study the application of gene-editing technologies. This review includes information related to tomato transformation methodology, one of the necessary requirements for gene editing, along with the status of site-directed mutagenesis by TALENs (transcription activator-like effector nucleases) and CRISPR/Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated Proteins). In addition to the reports on proof-of-concept experiments to demonstrate the feasibility of gene editing in tomato, there are many reports that show the power of these technologies for modification of traits, such as fruit characteristics (ripening, size, and parthenocarpy), pathogen susceptibility, architecture (plant and inflorescence), and metabolic engineering. Also highlighted in this review are reports on the application of a recent CRISPR technology called base editing that allows the modification of one base pair in a gene sequence and a strategy that takes advantage of a geminivirus replicon for delivery of DNA repair template.

  • CRISPR/Cas
  • site-directed mutagenesis
  • Solanaceae
  • Solanum lycopersicum
  • TALENs
  • transformation
  • Abbreviations

    BeYDV,
    bean yellow dwarf virus;
    CRISPR/Cas,
    clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins;
    DSB,
    double-strand break;
    HR,
    homologous recombination;
    indels,
    insertion and deletion;
    NHEJ,
    non-homologous end-joining;
    TALENs,
    transcription activator-like effector nucleases
    • Received July 19, 2017.
    • Revision received October 13, 2017.
    • Accepted October 16, 2017.
    • © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society and the Royal Society of Biology
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    Gene editing in tomatoes
    Joyce Van Eck
    Emerging Topics in Life Sciences Nov 2017, 1 (2) 183-191; DOI: 10.1042/ETLS20170056
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    Gene editing in tomatoes
    Joyce Van Eck
    Emerging Topics in Life Sciences Nov 2017, 1 (2) 183-191; DOI: 10.1042/ETLS20170056

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    Jump To

    • Article
      • Abstract
      • Introduction
      • Prerequisites for genome editing in tomato
      • TALEN-mediated genome editing of tomato
      • CRISPR/Cas9-mediated genome editing of tomato
      • Base editing via CRISPR/Cas9 in tomato
      • DNA repair mechanisms and DNA-repair template delivery into tomato cells
      • Conclusion
      • Funding
      • Competing Interests
      • References
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    Keywords

    CRISPR/Cas
    site-directed mutagenesis
    Solanaceae
    Solanum lycopersicum
    TALENs
    transformation

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